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Safety - Intraderm
Safety 2018-01-18T21:32:53+00:00

Impact of Microcyn® Technology on Fibroblast Cells as Compared to Brand V in Negative-Pressure Instillation Simulation

Study

An in-vitro study was conducted in which confluent monolayers of L-929 mouse fibroblast cells (as recommended by ISO and USP guidelines) were prepared and challenged by both Microcyn® Negative-Pressure Wound Therapy Solution and Brand V solution. The study was conducted on these fibroblast cells to evaluate potential toxicity with repeated exposure in-vitro. Results were recorded at one-hour intervals after four 15-second exposures (instillation), and later at 24 hours.
Microcyn NPWTi Solution samples were graded as non-toxic: Grade 1 to L-929 mouse fibroblast cells under the conditions of this study.The Brand V samples were graded as cytotoxic: Grade 3 to L-929 mouse fibroblast cells under the conditions of this study.After 24 hours, the results remained unchanged.

Results

Photos

The monolayers of fibroblast cells were graded using light microscopy. Cellular morphology was graded in accordance with the ISO acceptance criteria and USP guidelines.

Microcyn® Exposure:

As shown in microscopic photos numbered one and two, the fibroblast cells exposed to the Microcyn Technology maintained their viability and indicate a non-toxic level of exposure.

Photo 1: Representative Image of Microcyn Treatment at T=1. After First 15 sec Exposure to 1ml (5ml Microcyn:1ml MEM) of L-929 Cells. Photo taken an hour after exposure at 200X.

Photo 1: Representative Image of Microcyn Treatment at T=1. After First 15 sec Exposure to 1ml (5ml Microcyn:1ml MEM) of L-929 Cells. Photo taken an hour after exposure at 200X.

Photo 2: Representative Image of Microcyn Treatment at T=4. After Fourth 15 sec Exposure to 1ml (5ml Microcyn:1ml MEM) of L-929 Cells. Photo taken an hour after exposure at 200X.

Photo 2: Representative Image of Microcyn Treatment at T=4. After Fourth 15 sec Exposure to 1ml (5ml Microcyn:1ml MEM) of L-929 Cells. Photo taken an hour after exposure at 200X.

Brand V Exposure:

As shown in microscopic photos numbered 3 and 4, the fibroblast cells exposed to the Brand V solution after the fourth treatment did not maintain their viability and indicate a cytotoxic level of exposure. Notice the proliferation of round, non-viable cells in photo four.

Photo 3: Representative Image of Brand V Treatment at T=1. After First 15 sec Exposure to 1ml (5ml Brand V:1ml MEM) of L-929 Cells. Photo taken an hour after exposure at 200X.

Photo 3: Representative Image of Brand V Treatment at T=1. After First 15 sec Exposure to 1ml (5ml Brand V:1ml MEM) of L-929 Cells. Photo taken an hour after exposure at 200X.

Photo 4: Representative Image of Brand V Treatment at T=4. After Fourth 15 sec Exposure to 1ml (5ml Brand V:1ml MEM) of L-929 Cells. Photo taken an hour after exposure at 200X.

Photo 4: Representative Image of Brand V Treatment at T=4. After Fourth 15 sec Exposure to 1ml (5ml Brand V:1ml MEM) of L-929 Cells. Photo taken an hour after exposure at 200X.